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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
23/11/2022 |
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Data da última atualização: |
08/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARRAES, F. B. M.; VASQUEZ, D. D. N.; TAHIR, M.; PINHEIRO, D. H.; FAHEEM, M.; FREITAS-ALVES, N. S.; MOREIRA-PINTO, C. E.; MOREIRA, V. J. V.; PAES-DE-MELO, B.; LISEI-DE-SA, M. E.; MORGANTE, C. V.; MOTA, A. P. Z.; LOURENCO, I. T.; TOGAWA, R. C.; GRYNBERG, P.; FRAGOSO, R. da R.; ALMEIDA-ENGLER, J. de; LARSEN, M. R.; GROSSI-DE-SA, M. F. |
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Afiliação: |
FABRICIO B. M. ARRAES, FEDERAL UNIVERSITY OF RIO GRANDE DO SUL; DANIEL D. N. VASQUEZ, FEDERAL UNIVERSITY OF RIO GRANDE DO SUL; MUHAMMED TAHIR, UNIVERSITY OF SOUTHERN DENMARK; DANIELE H. PINHEIRO, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY; MUHAMMED FAHEEM, NATIONAL UNIVERSITY OF MEDICAL SCIENCES, PAKISTAN; NAYARA S. FREITAS-ALVES, FEDERAL UNIVERSITY OF PARANÁ; CLÍDIA E. MOREIRA-PINTO, CNPAE; VALDEIR J. V. MOREIRA, UNIVERSITY OF BRASÍLIA; BRUNO PAES-DE-MELO, CNPAE; MARIA E. LISEI-DE-SA, MINAS GERAIS AGRICULTURAL RESEARCH COMPANY; CAROLINA VIANNA MORGANTE, CPATSA; ANA P. Z. MOTA, INRAE; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; ROBERTO COITI TOGAWA, Cenargen; PRISCILA GRYNBERG, Cenargen; RODRIGO DA ROCHA FRAGOSO, CNPAE; JANICE DE ALMEIDA-ENGLER, INRAE; MARTIN R. LARSEN, UNIVERSITY OF SOUTHERN DENMARK; MARIA FATIMA GROSSI-DE-SA, Cenargen. |
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Título: |
Integrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Plants, v. 11, 2744, 2022. |
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ISSN: |
2223-7747 |
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DOI: |
https:// doi.org/10.3390/plants11202744 |
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Idioma: |
Inglês |
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Conteúdo: |
The root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10 overexpression can be effective against the plant parasitic nematodeM. incognita, but its mechanism of action remains unclear. These findings will help develop new engineered soybean genotypes with higher performance in response to RKN infections. MenosThe root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10... Mostrar Tudo |
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Palavras-Chave: |
Differential expression; Root-knot nematode. |
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Thesagro: |
Glycine Max; Meloidogyne Incognita; Soja. |
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Thesaurus Nal: |
Phenylpropanoids; Proteome; Transcriptome. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148619/1/Integrated-omic-approaches.pdf
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Marc: |
LEADER 03069naa a2200457 a 4500
001 2148619
005 2023-12-08
008 2022 bl uuuu u00u1 u #d
022 $a2223-7747
024 7 $ahttps:// doi.org/10.3390/plants11202744$2DOI
100 1 $aARRAES, F. B. M.
245 $aIntegrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions.$h[electronic resource]
260 $c2022
520 $aThe root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10 overexpression can be effective against the plant parasitic nematodeM. incognita, but its mechanism of action remains unclear. These findings will help develop new engineered soybean genotypes with higher performance in response to RKN infections.
650 $aPhenylpropanoids
650 $aProteome
650 $aTranscriptome
650 $aGlycine Max
650 $aMeloidogyne Incognita
650 $aSoja
653 $aDifferential expression
653 $aRoot-knot nematode
700 1 $aVASQUEZ, D. D. N.
700 1 $aTAHIR, M.
700 1 $aPINHEIRO, D. H.
700 1 $aFAHEEM, M.
700 1 $aFREITAS-ALVES, N. S.
700 1 $aMOREIRA-PINTO, C. E.
700 1 $aMOREIRA, V. J. V.
700 1 $aPAES-DE-MELO, B.
700 1 $aLISEI-DE-SA, M. E.
700 1 $aMORGANTE, C. V.
700 1 $aMOTA, A. P. Z.
700 1 $aLOURENCO, I. T.
700 1 $aTOGAWA, R. C.
700 1 $aGRYNBERG, P.
700 1 $aFRAGOSO, R. da R.
700 1 $aALMEIDA-ENGLER, J. de
700 1 $aLARSEN, M. R.
700 1 $aGROSSI-DE-SA, M. F.
773 $tPlants$gv. 11, 2744, 2022.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 9 | |
| 1. |  | GARCIA, R. A.; MACEDO, L. L. P.; NASCIMENTO, D. C. do; GILLET, F.-X.; MOREIRA-PINTO, C. E.; FAHEEM, M.; BASSO, A. M. M.; SILVA, M. C. M.; GROSSI-DE-SA, M. F. Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis. PLoS ONE, v. 12, n. 12, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | HABIB, P.; SOCCOL, C. R.; O'KEEFE, B. R.; KRUMPE, L. R. H.; WILSON, J.; MACEDO, L. L. P. de; FAHEEM, M.; SANTOS, V. O. dos; PRADO, G. S.; BOTELHO, M. A.; LACOMBE, S.; SA, M. F. G. de. Gene-silencing suppressors for high-level production of the HIV-1 entry inhibitor griffithsin in Nicotiana benthamiana. Process Biochemistry, v. 70, p. 45-54, 2018. Na publicação: Maria Fatima Grossi-de-Sa.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | FAHEEM, M.; VIDAL, J. F. D.; FERNANDES, J. P. C.; OLIVEIRA. G. M.; CAROLINE, C. F.; SOUTO, B. de M.; PARACHIN, N. S.; QUIRINO, B. F.; BARBOSA, J. A. R. G. Cloning and initial characterization of cellulolytic enzymes selected in a metagenomic approach. In: REUNIÃO ANUAL DA SBBQ, 42., 2013, Foz do Iguaçu, PR. [Anais...] São Paulo: SBBq, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 4. | | ARRAES, F. B. M.; ROCHA, S.; SÁ, D. M. de; FAHEEM, M.; MELO, B. P.; MORGANTE, C. V.; SOUZA JUNIOR, D.; NORIEGA, D.; SA, M. F. G. de; DANCHIN, E. G. In silico analysis reveal high variability in RNAi machinery of five different insect orders. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 5. | | LISEI-DE-SÁ, M. E.; ARRAES, F. B. N.; BRITO, G. G.; BENEVENTI, M. A.; LOURENCO-TESSUTTI, I.; BASSO, A. M. M.; AMORIM, R. M. S.; SILVA, M. C. M.; FAHEEM, M.; OLIVEIRA, N. G.; MIZOI, J.; YAMAGUCHI-SHINOZAKI, K.; GROSSI-DE-SA, M. F. AtDREB2A-CA influences root architecture and increases drought tolerance in transgenic cotton. Agricultural Sciences, v. 8, p. 1195-1225, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | FREITA, E. O.; MELO, B. P.; LOURENCO-TESSUTTI, I. T.; ARRAES, F. B. M.; AMORIM, R. M.; LISEI-DE-SÁ, M. E.; COSTA, J. A.; LEITE, A. G. B.; FAHEEM, M.; FERREIRA, M. A.; MORGANTE, C. V.; FONTES, E. P. B.; GROSSI-DE-SA, M. F. Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application. BMC Biotechnology, v. 19, article 79, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 7. | | FAHEEM, M.; MARTINS-DE-SÁ, D.; VIDAL, J. F. D.; ÁLVARES, A. C. M.; BRANDÃO-NETO, J.; BIRD, L. E.; TULLY, M. D.; DELFT, F. v.; SOUTO, B. de M.; QUIRINO, B. F.; FREITA, S. M.; BARBOSA, J. A. R. G. Functional and structural characterization of a novel putative cysteine protease cell wall-modifying multi-domain enzyme selected from a microbial metagenome. Scientific Reports, v. 6, n. 38031, 2016. p. 1-14.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | ARRAES, F. B. M.; MARTINS-DE-SA, D; VASQUEZ, D. D. N.; MELO, B. P.; FAHEEM, M.; MACEDO, L. L. P. de; MORGANTE, C. V.; BARBOSA, J. A. R. G.; TOGAWA, R. C.; MOREIRA, V. J. V.; DANCHIN, E. G. J.; SA, M. F. G. de. Dissecting protein domain variability in the core rna interference machinery of five insect orders. RNA Biology, v. 18, n. 11, p. 1653-1681, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 9. | | ARRAES, F. B. M.; VASQUEZ, D. D. N.; TAHIR, M.; PINHEIRO, D. H.; FAHEEM, M.; FREITAS-ALVES, N. S.; MOREIRA-PINTO, C. E.; MOREIRA, V. J. V.; PAES-DE-MELO, B.; LISEI-DE-SA, M. E.; MORGANTE, C. V.; MOTA, A. P. Z.; LOURENCO, I. T.; TOGAWA, R. C.; GRYNBERG, P.; FRAGOSO, R. da R.; ALMEIDA-ENGLER, J. de; LARSEN, M. R.; GROSSI-DE-SA, M. F. Integrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions. Plants, v. 11, 2744, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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